Z-VAD(OMe)-FMK Secrets
a OVCAR8 cells were being incubated with ten μM EdU, just before getting taken care of with vehicle, a hundred nM CX-5461, one hundred nM BMN-673 or the combination of both for 24 h. Co-IF for γH2AX and RAD51 was executed. Cells had been incubated for 30 minutes at place temperature with Click-IT reaction, washed with PBS after which co